ISO 21702:2019 pdf free
ISO 21702:2019 pdf free.Measurement of antiviral activity on plastics and other non-porous surfaces
Prepare the formaldehyde solution by adding 100 ml of 37 % formaldehyde solution to 900 ml of water.
If it is not intended to be used immediately after preparation, preserve it at 20 °C to 25 °C. Never use the formalin solution that has been kept for longer than one month after preparation.
NOTE The other solution for cell fixation can be used after appropriate validation for cell fixation.
Prepare the methylene blue solution by dissolving 0,375 g of the methylene blue and 62,5 μl of 1 N sodium hydroxide solutions in 1 000 ml of water. If it is not intended to be used immediately after preparation, then preserve it at 20°C to 25 °C. Never use the methylene blue solution that has been kept for longer than one month after preparation.
Put a freezed cryopreserved fetal bovine serum in a package into a water bath at 37 °C and keep it until it defrosts. Then, raise the temperature of the water bath to 56 °C and keep it for 30 min to inactivate.
Divide it into several tubes. Put them in a freezer at a temperature less than -20 °C. Just before using, put it in the water bath at 37 °C and keep it until it defrosts.
Dissolve 9,53 g of the Eagle’s minimum essential medium or 10,4 g of RPMI 1640 medium and 60 mg of kanamycin sulfate in 800 ml of water. Add water to make 1 000 ml of solution in total. Sterilize the solution by using a 0,22 um membrane filter.
When L-glutamine is not included in the EMEM or RPMI 1640 medium purchased in the market, sterilizing in the autoclave may be applied. Then, before use, add L-glutamine as listed in the example of composition in Annex A.
Add 15 ml of 7,5 % sodium bicarbonate solution and 100 ml of the inactivated fetal bovine serum in the solution and mix well. If it is not intended to be used immediately after preparation, then preserve it at 5 °C to 10 °C. Never use a growth medium that has been kept for longer than one month after preparation.ISO 21702 pdf download.
