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ISO 16221:2001 pdf free

ISO 16221:2001 pdf free.Water quality – Guidance for determination of biodegradability in the marine environment
ISO 16221 specifies five methods for determining the utimate aerobic biodegradability of organic compounds in the marine environment by aerobic microorganisms in static aqueous test systems. Standard degradation methods developed for testing in fresh water are modified and adapted to marine conditions. These methods are the DOC die-away test (ISO 7827), the closed bottle test (ISO 10707), the two-phase closed bottle test (ISO 10708), the CO2 evolution test (ISO 9439) and the CO2 headspace test (ISO 14593).
The methods apply to organic compounds which
a) are water-soluble under the conditions of the test used;
b) are poorly water-soluble under the conditions of the test used, in which case special measures may be necessary to achieve good dispersion of the compound (see for example, ISO 10634);
c) are volatile, provided that an appropriate test with suitable conditions is used;
d) are not inhibitory to the test microorganisms at the concentration chosen for the tests. The presence of inhibitory effects can be determined as specified in this International Standard.
NOTE The conditions described in this Intermational Standard do not always correspond to the optimal conditions for allowing the maximum degree of biodegradation to occur. For biodegradation methods in fresh water see ISO 14593 and ISO 15462, and for biodegradation at low concentrations see ISO 14592 .
The following normative documents contain provisions which, through reference in this text, constitute provisions of ISO 16221. For dated references, subsequent amendments to, or revisions of, any of these publications do not apply. However, parties to agreements based on this International Standard are encouraged to investigate the possbility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred to aplies. Members of ISO and IEC maintain registers of currently valid International Standards.
ISO 7827, Water quality 一Evaluation in an aqueous medium of the “ultimate” aerobic biodegradability of organic compounds一Method by analysis of dissolved organic carbon (DOC).
ISO 9439,Water quality 一Evaluation of ultimate aerobic biodegradbility of organic compounds in aqueous medium一Carbon dioxide evolution test.
ISO 10707, Water quality一Evaluation in an aqueous medium of the “ltmate”” aerobic biodegradability of organic compounds一Method by analysis of biochemical oxygen demand (closed bottle test).ISO 16221 pdf download.

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