BS ISO 27105:2016 pdf free

BS ISO 27105:2016 pdf free.Milk and cheese一Determination of hen’s egg white lysozyme content by high performance liquid chromatography
The values for repeatability and reproducibility derived from an interlaboratory test[7] were determined in accordance with ISO 5725-1[2] and ISO 5725-2.[3]
The values for the repeatability and the reproducibility limit are expressed for the 95 % probability level and may not be applicable to concentration ranges and matrices other than those given. Details of the interlaboratory test on the precision of the method are given in Annex B.
The absolute difference between two individual single test results, obtained with the same method on identical test material in the same laboratory by the same operator using the same equipment within a short interval of time, shall in not more than 5 % of the cases be greater than 7,7 % (95 % confidence intervals 6,2 % to 8,5 %).
The absolute difference between two individual single test results, obtained with the same method on identical test material in different laboratories with different operators using different equipment, shall in not more than 5 % of the cases be greater than 33,8 % (95 % confidence intervals 20,3 % to 57,5 %).
Running the chromatographic system on another temperature, using a different column type, or other differing conditions do not result automatically in false or less sensitive results but need thoroughly to be validated.
An international collaborative test according to ISO/TS 27105|IDF/RM 216:2009 involving 20 laboratories was carried out on seven samples oflysozyme in milk and cheese from Italy and Switzerland.Two laboratories did not send results and three did not respect the ISO/TS 27105|IDF/RM 216:2009.Four of the samples contained no measurable amount of analyte. They were correctly identified by the laboratories as such and used to calculate the detection limit and selectivity. One laboratory showed a significant bias and was excluded from the statistical evaluation. Observed homogeneity in individual precision values allowed the calculation of precision values r and R for the concentration ranges in Table B.1, which are recommended for use.
Table B.1 shows the results for different samples and concentration ranges. The values were calculated on the basis of the mean values of the single samples reported by the laboratories.BS ISO 27105 pdf free.